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KMID : 0363320120330040438
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Journal of Korean Oriental Internal Medicine
2012 Volume.33 No. 4 p.438 ~ p.447
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Effects of Ethanol Extract of Benincasa Seeds on the Experimental Cellular Model of Nonalcoholic Fatty Liver Disease
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Choi Jun-Yong
Kim So-Yeon
Kwun Min-Jung
Kim Kyun-Ha
Joo Myung-Soo
Han Chang-Woo
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Abstract
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Objectives : In this study, we investigated the effect and the underlying mechanism of ethanol extract of Benincasa seeds
on a cellular model of non-alcoholic fatty liver disease (NAFLD) established by treating HepG2 cells with palmitate.
Methods : We evaluated ethanol extract of Benincasa seeds (EEBS) for its hepatic lipid-lowering potential in fatty acid
overloaded HepG2 cells. After incubation in palmitate containing media with or without EEBS, intracellular neutral lipid
accumulations were quantified by Nile red staining. We also investigated the effect of EEBS on lipogenesis and β-oxidation. LXRα-dependent SREBP-1c activation, expression of lipogenic genes, and expression of β-oxidation related genes were determined with or without pretreatment of EEBS.
Results : EEBS significantly attenuated palmitate-induced intracellular neutral lipid accumulation in HepG2 cells. EEBS
suppressed fatty acid synthesis by inhibiting LXRα-dependent SREBP-1c activation. EEBS also repressed SREBP-1c mediated induction of lipogenic genes, including ACC, FAS, and SCD-1. However, EEBS had no effect on β-oxidation related CPT-1 and PPARα gene expression.
Conclusions : Our results suggest that EEBS has an efficacy to decrease hepatic lipid accumulation, and this effect was
mediated by inhibiting the LXRα-SREBP-1c pathway that leads to expression of lipogenic genes and hepatic steatosis.
Therefore, the Benincasa seeds may have a potential clinical application for treatment of this chronic liver disease.
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KEYWORD
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Benincasa seed, NAFLD, LXRα, SREBP-1c
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